| Format: Immunoblot | | Detection Method: Immunoblot | | Species Reactivity: bovine, human, mouse, rat | | Assay Time: 5.5-6 h | | Sample Type: Cell extracts | | Kit Contents: A Control Blocking Peptide, Uninduced Immunoblotting Standard, Induced Immunoblotting Standard, PARP Immunoblotting Standard, and a user protocol. | | Comments: An assay kit designed to detect poly(ADP-ribose) polymerase (PARP) cleavage by immunoblotting. PARP is an enzyme implicated in DNA damage and repair mechanisms. During apoptosis, PARP is cleaved by caspase-3. Cleavage of PARP from the native 116 kDa to 85 kDa is a hallmark of apoptosis. Each kit is provided with a highly specific rabbit polyclonal antibody that detects 116 kDa PARP and the 85 kDa apoptosis-related cleavage fragment from human, bovine, rat, and mouse. | | Ref.: Duriez, P.J., et al. 1997. Biochim. Biophys. Acta 1334, 65. Lazebnik, Y.A., et al. 1994. Nature 371, 346. Kaufman, S., et al. 1993. Cancer Research. 53, 3976. Lamarre, D., et al. 1988. Biochim. Biophys. Acta 950, 147. | | R: 20/22-24-36/37/38-40-41; S: 26-36/37/39-45-53 |
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