rPMT Liquid. In 50 mM Tris-HCl, 10% glycerol, pH 7.5. AVOID FREEZE/THAW CYCLES. Recombinant Pasteurella multocida toxin expressed in E. coli. A bacterial protein toxin that binds to and enters eukaryotic cells via receptor-mediated endocytosis. Acts intracellularly to initiate the inositol trisphosphate signaling pathway, Ca2+ mobilization, and DNA synthesis. Stimulates tyrosine phosphorylation of multiple substrates including focal adhesion kinase. Exerts its action on the a-subunit of the Gq family of G-proteins. Has been used for the activation of Gq-protein-coupled phosphatidylinositol-specific phospholipase C (PLC) in intact cells, making PMT useful as a tool for studying Gq-linked signal transduction. Purity: ≥95% by SDS-PAGE. CAS 129876-91-1. Ref.: Wilson, B.A., et al. 1997. J. Biol. Chem. 272, 1268. Lacerda, H.M., et al. 1996. J. Biol. Chem. 271, 439. Higgins, T.E., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 4240. Murphy, A.C., and Rozengurt, E. 1992. J. Biol. Chem. 267, 25296.
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