Lyophilized solid. Lyophilized from 100 mM NaCl, 30 mM Tris, 0.1% sucrose, pH 8.3. A bacterial protein toxin that binds to and enters eukaryotic cells via receptor-mediated endocytosis. Acts intracellularly to initiate the inositol trisphosphate signaling pathway, Ca2+ mobilization, and DNA synthesis. Stimulates tyrosine phosphorylation of multiple substrates including focal adhesion kinase. Exerts its action on the a-subunit of the Gq family of G-proteins. Has been used for the activation of Gq-protein-coupled phosphatidylinositol-specific phospholipase C (PLC) in intact cells, making PMT useful as a tool for studying Gq-linked signal transduction. Purity: Single band by SDS-PAGE. CAS 129876-91-1. Ref.: Seo, B., et al. 2000. J. Biol. Chem. 275, 2239. Essler, M., et al. 1998. J. Immunol. 161, 5640. Wilson, B.A., et al. 1997. J. Biol. Chem. 272, 1268. Lacerda, H.M., et al. 1996. J. Biol. Chem. 271, 439. Foged, N.T. 1992. Acta Pathol. Microbiol. Immunol. Scand. 100 (Suppl. 25), 1. Staddon, J.M., et al. 1991. J. Biol. Chem. 266, 4840. Buys, W.E., et al. 1990. Nucleic Acids Res. 18, 2815. Lax, A.J., et al. 1990. FEBS Lett. 227, 59. Petersen, S.K. 1990. Mol. Microbiol. 4, 821. Foged, N.T., et al. 1987. FEMS Microbiol. Lett. 43, 45.
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