Peroxisome Proliferator-Activated Receptor, a-isotype

Liquid. In 100 mM KCl, 20 mM Tris-HCl, 0.2 mM EDTA, 1 mM DTT, 20% glycerol, pH 8.0. AVOID FREEZE/THAW CYCLES. Recombinant, human PPARa expressed in E. coli. Belongs to the family of peroxisome proliferator-activated receptors (PPARs), the nuclear hormone receptors that are activated by fatty acids and derivatives. PPARa is expressed most in brown adipose tissue and liver, and to some extent in the kidney, heart, and skeletal muscle. The target genes of PPARa are a relatively homogeneous group of genes that participate in aspects of lipid catabolism such as fatty acid uptake through membranes, fatty acid binding in cells, fatty acid oxidation (in microsomes, peroxisomes, and mitochondria) and lipoprotein assembly and transport. One unit is equal to 1 ng of purified protein. 20 units are sufficient for a gel-mobility shift assay and 100 units are sufficient for a protein-protein interaction assay. Purity: ≥95% by SDS-PAGE. Contaminants: DNase, RNase, and protease activity: none detected.

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