nhPDGF Lyophilized. Lyophilized from sterile-filtered 25% acetonitrile, 0.1% TFA. Native platelet-derived growth factor from human platelets. Consists of approximately 70% PDGF-AB and 30% PDGF-BB. PDGF action is mediated by cell surface a- and b-receptors. M.W. 28,000-32,000. Biological activity: ED50 = 1-3 ng/ml as measured in a mitogenic assay using quiescent NR 6-3T3 fibroblasts. Purity: ≥97% by SDS-PAGE. Contaminants: Endotoxin: ≤1 EU/µg PDGF. Prepared from blood that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV. Merck Index: 14, 7525
Ref.: Abboud, H.E., et al. 1994. J. Cell Physiol. 158, 140. Soma, Y., et al. 1994. Exp. Cell Res. 212, 274. Hammacher, A., et al. 1988. J. Biol. Chem. 263, 16493. Raines, E.W., et al. 1985. Methods Enzymol. 109, 749. |
