rhPDGF-BB Lyophilized. Lyophilized from a sterile-filtered solution, carrier free. Recombinant, human platelet-derived growth factor (disulfide-linked dimer of two 109 amino acid B-chain monomers) expressed in E. coli. Shown to be a potent chemoattractant for neutrophils, mesenchymal and mononuclear cells. Has the same amino acid sequence as mature human PDGF-B. Rapidly activates protein kinase D in vascular smooth muscle cells. Biological activity: ED50 = 1-5 ng/ml as determined by the dose dependent proliferation of BALB/c 3T3 cells. Purity: ≥95% by SDS-PAGE. Contaminants: Endotoxin: ≤1 ng/µg PDGF-BB. Merck Index: 14, 7525
Ref.: Abedi, H., et al. 1998. FEBS Lett. 427, 209. Abboud, H.E., et al. 1994. J. Cell Physiol. 158, 140. Westermark, B., and Heldin, C.-H. 1991. Cancer Res. 51, 5087. Raines, E.W., et al. 1985. Methods Enzymol. 109, 749. Johnson, A., et al. 1984. EMBO J. 3, 921. |
