PDEII

Suspension. In 3.2 M amounium sulfate, 50 mM ammonium acetate, 50 mM NaCl, 0.1% Tween^®-80 Detergent, pH 6.0. DO NOT FREEZE. Native phosphodiesterase type II from calf spleen. Useful in sequence studies of oligonucleotides derived from RNA and DNA. Attacks the 5′-terminal end of phosphodiester bonds, releasing 3′-mononucleotides. Specific activity: ≥7 units/mg protein. One unit is defined as the amount of enzyme that will produce 1 µmol of 3′-thymidine-phosphate per min at 25°C after hydrolysis of thymidylyl(3′→5′)thymidine (TpT). Dilute in deionized water just before use. Contaminants: Phosphomonoesterase, 3'-phosphodiesterase: none detected. pH optimum 6.0. EC 3.1.16.1, CAS 9068-54-6.

Ref.: Gibson, L.C.D., et al. 2006. Eur. J. Pharmacol. 538, 39. Bentzley, C.M., et al. 1998. Anal. Biochem. 258, 31. Bernardi, A., and Bernardi, G. 1971. Enzymes 4, 329. Ipata, P., and Felicioli, R.A. 1969. Eur. J. Biochem. 8, 174.