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  Phosphodiesterase 4B2, Catalytic Domain, His•Tag®, Human, Recombinant, S. frugiperda
Cat. No. 524732  
All Categories » Calbiochem » Proteins and Enzymes » Phosphodiesterases

cAMP-specific phosphodiesterase 4B2, Catalytic Domain
PDE4B2CAT

Liquid. In 150 mM NaCl, 10 mM Tris, 0.1% NP-40, pH 8.3. AVOID FREEZE/THAW CYCLES. Recombinant, human phosphodiesterase 4B2 catalytic domain fused to a His•Tag® sequence and expressed in S. frugiperda insect cells using a baculovirus expression system. Supplied as a partially purified preparation. Activity: ≥0.5 units/µl. One unit is defined as the amount of enzyme that will convert 1 µmole cAMP to AMP per min at pH 7.5, 30°C.

Ref.: Gibson, L.C.D., et al. 2006. Eur. J. Pharmacol. 538, 39. Xu, R.X., et al. 2000. Science 288, 1822.

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Phosphodiesterase 4B2 was treated with increasing concentration of the PDE inhibitor, Rolipram (Cat. No. 557330), and the activity assayed. The IC50 for this inhibition is 2.53 µM.

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Material Safety Data Sheets:
524732: Phosphodiesterase 4B2, Catalytic Domain, His•Tag®, Human, Recombinant, S. frugiperda - English
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All Categories » Calbiochem » Proteins and Enzymes » Phosphodiesterases