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  Phosphodiesterase 5, Catalytic Domain, Human, Recombinant, S. frugiperda
Cat. No. 524738  
All Categories » Calbiochem » Proteins and Enzymes » Phosphodiesterases

cGMP-Specific Phosphodiesterase 5, Catalytic Domain
PDE5CAT

Liquid. In 150 mM NaCl, 10 mM Tris-HCl, 0.1% NP-40, pH 8.3. AVOID FREEZE/THAW CYCLES. Recombinant, human, phosphodiesterase 5 catalytic subunit expressed in S. frugiperda insect cells using a baculovirus expression system. Supplied as a partially purified, catalytically active preparation. Activity: ≥ 0.5 units/µl. One unit is defined as the amount of enzyme that will convert 1 pmol cGMP to GMP per min at 30°C, pH 7.5.

Ref.: Gibson, L.C.D., et al. 2006. Eur. J. Pharmacol. 538, 39. Blount, M.A., et al. 2004. Mol. Pharmacol. 66, 144. Bakre, M.M., et al. 2000. J. Cell. Biochem. 77, 159.

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Phosphodiesterase 5 was treated with increasing concentrations of the inhibitor, sildenafil, and the activity assayed. the IC50 for this inhibition is 49 nM.

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All Categories » Calbiochem » Proteins and Enzymes » Phosphodiesterases