cGMP-Specific Phosphodiesterase 5, Catalytic Domain PDE5CAT Liquid. In 150 mM NaCl, 10 mM Tris-HCl, 0.1% NP-40, pH 8.3. AVOID FREEZE/THAW CYCLES. Recombinant, human, phosphodiesterase 5 catalytic subunit expressed in S. frugiperda insect cells using a baculovirus expression system. Supplied as a partially purified, catalytically active preparation. Activity: ≥ 0.5 units/µl. One unit is defined as the amount of enzyme that will convert 1 pmol cGMP to GMP per min at 30°C, pH 7.5. Ref.: Gibson, L.C.D., et al. 2006. Eur. J. Pharmacol. 538, 39. Blount, M.A., et al. 2004. Mol. Pharmacol. 66, 144. Bakre, M.M., et al. 2000. J. Cell. Biochem. 77, 159. |