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  Phosphodiesterase 1A3, Human, Recombinant, S. frugiperda
Cat. No. 524740  
All Categories » Calbiochem » Proteins and Enzymes » Phosphodiesterases

Hcam1
PDE1A3

Liquid. RIPA buffer (150 mM NaCl, 10 mM Tris, 0.1% NP-40), pH 8.3 AVOID FREEZE/THAW CYCLES. Full-length, recombinant, human phosphodiesterdase 1A3 expressed in S. frugiperda insect cells using a baculovirus expression system. Supplied as a partially purified preparation of catalytically active enzyme. Activity: ≥0.5 units/µl. One unit is defined as the amount of enzyme that will convert 1 pmol cAMP to AMP per min at 30°C, pH 7.5.

Ref.: Gibson, L.C.D., et al. 2006. Eur. J. Pharmacol. 538, 39. Fidock, M., et al. 2002. Cell Signal. 14, 53. Loughney, K., et al. 1996. J Biol Chem. 271, 796.

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Phosphodiesterase 1A3 was treated with increasing concentrations of the inhibitor, 8Me-IBMX (Cat. No. 454202) and the activity determined. The IC50 for this inhibition is 6.04 µM.

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All Categories » Calbiochem » Proteins and Enzymes » Phosphodiesterases