Silent mating type information regulation 2 homolog
Sirtuin1, GST-Fusion, Human, Recombinant, E. coli Liquid. In 138 mM NaCl, 50 mM Tris-HCl, 20 mM glutathione, 10% glycerol, pH 8.0. AVOID FREEZE/THAW CYCLES. Recombinant, human SIRT1 histone deacetylase consisting of amino acids 193-741 fused at the N-terminus to GST and expressed in E. coli. SIRT1 preferentially deacetylates histones H4 at K16 and H3 at K9 and interacts with and deacetylates histone H1 at K26. More than a dozen non-histone proteins have been found to serve as substrates for SIRT1. It plays a role in several biological processes including stress resistance, metabolism, differentiation, and aging. The active deacetylase is useful in the study of SIRT1 regulation and for inhibitor screening. Specific activity: ≥8 units/µg protein. One unit is defined as the amount of enyme required to deacetylate 1 pmol fluorescent peptide substrate [Arg-His-Lys-Lys(Ac)], per min at 30°C. Purity: ≥80% by SDS-PAGE. Ref.: Kolthur-Seetharam, U., et al. 2006. Cell Cycle 5, 873. Solomon, J.M., et al. 2006. Mol. Cell. Biol. 26, 28. |
