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| | PhosphoDetect™ Anti-Phosphoserine Mouse mAb (16B4) |
|  | Cat. No. 525280 |
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| Host: Mouse | | Isotype: IgM | | Immunogen: a mixture of phosphopeptides containing phosphorylated serine, conjugated to KLH | | Form: Lyophilized | | Formulation: 50 µg antibody lyophilized from 2X PBS, PEG, sucrose and 150 µl control lysate lyophilized from 2X PBS, PEG, sucrose, 10 mM NaF. | | Preservative: ≤0.1% sodium azide (antibody and control lysate) | | Comments: Recognizes a broad range of serine-phosphorylated proteins in crude extracts from multiple species, requiring either proline or lysine C-terminal to the phosphoserine. Supplied with a positive control. |
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EMD Chemicals Inc. USD list price is displayed (pricing with local distributors may vary). NOTE: In Stock status is based on item availability worldwide.
| 16B4 | a broad range of species | ELISA, IB, IP
See Key |
Detection of phosphoserine-containing proteins by immunoblotting. Sample: Whole cell lysate from A431 cells treated with pervanadate (all lanes). Primary antibody: Anti-Phosphoserine Mouse mAb (16B4) (Cat. No. 525280) (1 µg/ml). Detection: chemiluminescence. Available separately: Anti-Phosphoserine (1C8) (Mouse) (Cat. No. 525281), Anti-Phosphoserine (4A3) (Mouse) (Cat. No. 525283), and Anti-Phosphoserine (4A9) (Mouse) (Cat. No. 525284), and Phosphoserine Detection Kit (Cat. No. 525282).
Detection of phosphoserine-containing antibodies by immunoblotting. Samples: Extract from rabbit muscle*. Primary antibody: Anti-Phosphoserine Mouse mAb (16B4) (Cat. No. 525280) (1 µg/ml). Detection: chemiluminescence. *Positive control supplied with the antibody. Available separately: Anti-Phosphoserine (1C8) (Mouse) (Cat. No. 525281), Anti-Phosphoserine (4A3) (Mouse) (Cat. No. 525283), and Anti-Phosphoserine (4A9) (Mouse) (Cat. No. 525284), and Phosphoserine Detection Kit (Cat. No. 525282).
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