Liquid. In 100 mM NaCl, 10 mM HEPES, 2.5 mM MgCl2, 20% glycerol, pH 7.5. AVOID FREEZE/THAW CYCLES. Recombinant, human phosphoinositide 3-kinase composed of the active catalytic p110b subunit and the regulatory p85 a subunit and expressed in S. frugiperda insect cells. PI 3-Kb is a ubiquitously expressed enzyme that is activated by insulin signaling or GPCR. The b isoform has been shown to regulate both cell growth and apoptotic responses. The active complex is useful for the study of activity regulation and inhibitor screening. MW: 83500 (p85a) and 124300 (p110b). Specific activity: ≥3 nmol/min/mg protein. Activity is measured as nmol phosphate transferred per min using phosphatidylinositol as the substrate. Purity: ≥90% by SDS-PAGE. Ref.: Vogt, P.K., et al. 2006. Virology 344, 131. |
