Liquid. In 100 mM NaCl, 10 mM HEPES, 2.5 mM MgCl2, 20% glycerol, pH 7.5. AVOID FREEZE/THAW CYCLES. Recombinant, human phosphoinositide 3-kinase p110d/p85a composed of the active catalytic p110 d isoform fused to GST coexpressed with the regulatory p85a subunit in S. frugiperda insect cells. Largely expressed in haematopoietic cells, the d isoform has been shown to be required for stem cell signaling and survival. The active complex is useful for the study of the regulation of activity and inhibitor screening. MW: 83500 (p85a) and 120200 (p110d, without GST). Ref.: Vogt, P.K., et al. 2006. Virology 344, 131. |
