RhoB-GS160-169
Rhodamine B-QRLFQVKGRR-OH Pinkish red lyophilized solid. Supplied as a trifluoroacetate salt. PROTECT FROM LIGHT. HYGROSCOPIC. PACKAGED UNDER INERT GAS. A Rhodamine-B-labeled decapeptide derived from the (PIP2)-binding region in segment-2 of gelsolin. Acts as a cell-permeable, reversible, and non-toxic agent that transiently disrupts actin filaments, which are essential for cell morphology and cell motility (~1-10 µM). Specifically binds to polyphosphoinositide (PPI) with high affinity and activates platelet without significantly affecting intracellular Ca2+ influx. Undergoes a reduction in fluorescence intensity upon binding to phosphatidylinositol 4,5-bisphosphate (PIP2) and lysophosphatidic acid; the fluorescence is not affected in the presence of phosphatidylserine and phosphatidylcholine. Useful fluorescent probe for the cellular localization and stabilization of PPIs. Purity: ≥95% by HPLC. Excitation max.: 565 nm, Emission max.: 590 nm Rho-B-Gln-Arg-Leu-Phe-Gln-Val-Lys-Gly-Arg-Arg-OH Ref.: Bucki, R., et al. 2001. Biochemistry 40, 15752 Cunningham, C.C., et al. 2001. J. Biol. Chem. 276, 43390. |
