Dark red solid. PROTECT FROM LIGHT. Membrane impermeable DNA intercalator. Has red fluorescence at 488 nm. Useful for flow cytometry for staining apoptotic cells and nuclei. Can be used to differentiate between apoptotic and necrotic cell death while stains only necrotic cells. Can be used together with common green fluorescent probes labeled with fluorescein isothiocyanate (FITC). Excitation max.: ~536 nm, Emission max.: ~617 nm Extinction coefficient(493 nm): 5900 M -1cm-1;(287 nm): 55,700 M -1cm-1; RTECS SF7949600, CAS 25535-16-4. Ref.: Belloc, F., et al. 1994. Cytometry 17, 59. Crompton, T., et al. 1992. Biochem. Biophys. Res. Commun. 183, 532. Darzynkiewicz, Z., et al. 1992. Cytometry 13, 795. de Caestecker, M.P., et al. 1992. J. Immunol. Methods 154, 11. Pollice, A.A., et al. 1992. Cytometry 13, 432.
R: 36/37/38-46; S: 22-26-36/37/39-45 |
