Liquid. In 150 mM NaCl, 50 mM HEPES, 1 mM DTT, pH 7.6. AVOID FREEZE/THAW CYCLES. Native, rabbit 20S proteasome. Catalytic core enzyme of the 26S proteasome that degrades ubiquitinated proteins. Hydrolyzes various peptide substrates and proteins with broad specificity in a non-ATP-dependent process. Can be activated by PA28 (Cat. No. 506280) or SDS. Biological activity: Fully functional as measured using Z-LLVY-AMC as the substrate following SDS activation. Purity: ≥95% by SDS-PAGE. Ref.: Stein, R.L., et al. 1996. Biochemistry 35, 3899. Ciechanover, A. 1994. Cell 79, 13. Dick, L.R., et al. 1994. J. Immunol. 152, 3884. Dick, L.R., et al. 1991. Biochemistry 30, 2725. Hough, R., et al. 1987. J. Biol. Chem. 262, 8303. |
