Format: Cuvette or 96-well plate

Detection Method: Fluorometric

Assay Time: 30 min

Kit Contents: 20S Proteasome in Buffer, Reaction Buffer, SDS, Substrate Solution, and a user protocol. Does not include the 7-Amino-4-methylcoumarin (AMC; Cat. No. 164545) standard.

Comments: Useful in quantifying 20S proteasome activity in cuvettes or a ½ volume 96-well plate. 20S activity is measured by monitoring the release of free AMC from the fluorogenic peptide Suc-Leu-Leu-Val-Tyr-AMC over time.

Ref.: DeMartino, G.N., and Slaughter, C. 1999. J. Biol. Chem. 274, 22123. Adams, G., et al. 1998. Biochemistry 37, 12927. Glickman, M., et al. 1998. Cell 94, 615. Groll, M., et al. 1997. Nature 386, 463. Coux, O., et al. 1996. Annu. Rev. Biochem. 65, 801. Stein, R. et al. 1996. Biochemistry 35, 3899. Seemuller, E., et al. 1995. Science 268, 579. Shibatani, T., and Ward, W. 1995. Arch. Biochem. Biophys. 321, 160. Hoffman, L., et al. 1992. J. Biol. Chem. 267, 22362. Ma, C.P., et al. 1992. J. Biol. Chem. 267, 10515.

R: 36/37/38-22; S: 26-36