Format: 1.0 ml bead suspension

Species Reactivity: canine, human, mouse

Assay Time: 3-5 h

Sample Type: Cell extracts

Kit Contents: UbL Beads Sufficient to process 12.5 to 25 mg of Lysate, Control Glutathione-Agarose Beads, Control Lysate, and a user protocol.

Comments: A rapid method for the isolation of biologically active proteasomes using affinity matrix beads comprised of a GST-fusion protein containing an ubiquitin-like domain (UbL) bound to GST-agarose. The proteasome subunit proteins can be identified by loading the beads directly onto an SDS-PAGE gel and immunoblotting with subunit specific antibodies. Alternatively, proteasome bound to beads can be used in proteolytic assays using proteasome substrates.

Ref.: Chen, L., and Madura, K. 2002. Mol. Cell Biol. 22, 4902. Chen, L., et al. 2001. EMBO Rep. 2, 933. Schauber, C., et al. 1998. Nature 391, 715.

Sold under license of U.S. Patent 6,294,363.