cAMP-Dependent Protein Kinase, Catalytic Subunit
Protein Kinase A, Catalytic Subunit Liquid. In 50 mM NaCl, 20 mM Tris-HCl, 1 mM EDTA, 2 mM DTT, 50% glycerol, pH 7.5. AVOID FREEZE/THAW CYCLES. Recombinant, mouse PKA catalytic subunit expressed in E. coli. Regulates a number of cellular functions including gene expression, carbohydrate metabolism, Ca2+ homeostasis, and ion channel function. Suitable for studying the phosphorylation of purified substrates and for use with cell extracts. Unlike the holoenzyme, the catalytic subunit does not require cAMP for activation. Activity: ≥2.5 x 106 U/ml. Specific activity: ≥5 x 106 U/mg protein. One unit is defined as the amount of enzyme that will transfer 1.0 pmol phosphate to Kemptide (LRRASLG; Cat. No. 05-23-4900) per min at 30°C, pH 7.5. Contaminants: Phosphatases, proteases: none detected. EC 2.7.1.37. Ref.: Grant, B.D., and Adams, J.A. 1996. Biochemistry 35, 2022. Lee, S-L. and Steinberg, R.A. 1996. J. Biol. Chem. 271, 16553. Adams, J.A., et al. 1995. Biochemistry 34, 2447. Bosma, M.M., et al. 1993. J. Neurosci. 13, 5242. Brindle, P., et al. 1993. Nature 364, 821. Hajnoczky, G., et al. 1993. Biochem. J. 293, 413. Sculptoreanu, A., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 10135. Wang, L-Y., et al. 1993. Science 259, 1173. Slice, L.W., and Taylor, S.S. 1989. J. Biol. Chem. 264, 20940. Uhler, M.D., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 1300. Walsh, D.A., et al. 1968. J. Biol. Chem. 243, 3763. |
