cAMP Dependent Protein Kinase
Protein Kinase A

Liquid. In 150 mM KCl, 30 mM K₂HPO₄, 1 mM DTT, 1 mM EDTA, 50% glycerol, pH 7.4. AVOID FREEZE/THAW CYCLES. Recombinant, human, catalytic subunit of PKA fused to at the N-terminus to a His•Tag^® sequence and expressed in E.coli. PKA regulates a number of cellular functions including gene expression, carbohydrate metabolism, Ca²⁺ homeostasis, and ion channel function. Suitable for labeling PKA substrates. Specific activity: ≥ 500,000 units/mg protein. One unit is defined as the amount of enzyme required to transfer 1 pmol phosphate to PKA peptide (GRTGRRNSI) per min at 30°C. Purity: ≥95% by SDS PAGE.

Ref.: Skalhegg, B.S. and Tasken K., et al. 2000. Front. Biosci. 5, D678.