| Format: 96-well plate | | Form: 96 Tests | | Detection Method: Colorimetric | | Sample Type: Cell lysates, purified enzymes | | Kit Contents: 96-Well Substrate-Coated Plate, Sample Preparation Buffer, Reaction Buffer, Calcium Solution, Phosphatidylserine, Detector Antibody, Streptavidin-HRP, Wash Buffer, Substrate Solutions, Stop Solution, and a user protocol. | | Comments: Useful for measuring protein kinase A (PKA) or protein kinase C (PKC) activity in crude cell extracts or semi-purified enzyme preparations. The assay kit is based on an ELISA method which utilizes a synthetic PKA/PKC pseudosubstrate and a monoclonal antibody which recognizes the phosphorylated form of that peptide. May be used for screening inhibitors and activators of PKA and/or PKC. Supplied with a user protocol. | | Ref.: Newton, A.C. 1995. J. Biol. Chem. 270, 28495. Nishizuka, Y. 1995. FASEB J. 9, 484. Nishizuka, Y. 1988. Nature 334, 661. Edelman, A.M., et al. 1987. Ann. Rev. Biochem. 56, 567. Nishizuka, Y. 1986. Science 233, 305. Montimity, M.R. and Bilezikijan, L.M. 1987. Nature 56, 175. Inagaki, M., et al. 1985. J. Biol. Chem. 260, 2922. Wolf, M., et al. 1985. Nature 317, 546. Beavo, J.A., et al 1974. Methods Enzymol. 38, 299. | | R: 20/21/22-34-36/37/38-42/43-45; S: 26-36/37/39-45-3-28 | | Not available for sale in Japan. |
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