Protein Phosphatase 2A₂

Liquid. In 25 mM Tris-HCl, 14 mM bME, 1 mM benzamidine, 100 µM EDTA, 100 µM PMSF, 50% glycerol, pH 7.0. Native, PP2A₂ from bovine kidney. A divalent cation-independent protein serine/threonine phosphatase involved in the control of numerous cellular processes. Has a catalytic subunit of 36 kDa and a regulatory subunit of 60 kDa. Transient phosphorylation of the catalytic subunit on threonine or tyrosine residues has been reported to inactivate PP2A₂. Inhibits nuclear telomerase activity in human breast cancer cells. Specific activity: ≥750 units/mg. One unit is defined as the amount of enzyme that will release 1.0 nmol of [³²P]P_i from ³²P-labeled phosphorylase per min at 30°C, pH 7.0. Purity: ≥90% by SDS-PAGE. EC 3.1.3.16.

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