Liquid. In 50 mM Tris-HCl, 14 mM b-mercaptoethanol, 1 mM benzamidine, 1 mM EDTA, 100 µM PMSF, 10% glycerol, 0.1% BRIJ®-35 detergent, pH 7.4. AVOID FREEZE/THAW CYCLES. Recombinant, human PP2AI I1 fused to GST and expressed in E. coli. Acts as a potent, heat-stable, specific, and non-competitive inhibitor of PP2A (Ki = 30 nM with myelin basic protein, histone H1, or phosphorylase as substrates for PP2A). Does not inhibit PP1, PP2B, or PP2C, or pyruvate dehydrogenase phosphatase. Purity: ≥90% by SDS-PAGE. Ref.: Li, M., et al. 1996. Biochemistry 35, 6998. Li, M., et al. 1995. Biochemistry 34, 1988. |
