cAMP-Dependent Protein Kinase Catalytic Subunit
Protein Kinase A, Catalytic Subunit Liquid. In 350 mM K2HPO4, 100 µM DTT, pH 6.8. AVOID FREEZE/THAW CYCLES. Native PKA catalytic subunit purified from bovine heart. The catalytic subunit of PKA dissociates from the PKA holoenzyme following binding of cAMP to the PKA regulatory subunits and phosphorylates target proteins at serine and/or threonine within the recognition motif, Arg-Arg-X-Ser*-X. The purified monomeric catalytic subunit does not require cAMP for activation and is suitable for studies of PKA phosphorylation in vitro with purified substrates or following its introduction into intact cells or cell extracts. Specific activity: ≥20,000 units/µg protein. One unit is defined as the amount of enzyme required to incorporate 1 pmol phosphate into Kemptide substrate (Cat. No. 05-23-4900) per min at 30°C. Purity: ≥95% by SDS-PAGE. Note: 25 µg= 500,000 units. Ref.: Corbin, J.D., et al. 1988. Methods Enzymol 159, 74. Rannels, S.R., et al 1983. Methods Enzymol 99, 55. Witt, J.J. and Roskoski, R. Jr. 1975. Anal Biochem. 66, 253. |
