cGMP-Dependent Protein Kinase Catalytic Subunit
PKGIa Catalytic Subunit
Protein Kinase G Ia Liquid. In 150 mM NaCl, 25 mM b-mercaptoethanol, 10 mM K2HPO4, 1 mM EDTA, 12% sucrose, pH 6.8. AVOID FREEZE/THAW CYCLES. Native PKGIa purified from bovine lung. PKG is involved in regulation of smooth muscle relaxation, platelet function, and cell division. PKG is activated by binding of cGMP to regulatory subunits within the holoenzyme, but in contrast to PKA, the activation of PKG does not invlove physical dissociation of catalytic from the regulatory domains. The activated enzyme phosphorylates target proteins at serine and/or threonine residues that are found within the recognition motif, Arg-Lys-Arg-Ser*-Arg-Ala-Glu. Specific activity: ≥1300 units/µg enzyme. One unit is defined as the amount of enzyme required to incorporote 1 pmol phosphate into the synthetic heptapeptide substrate, RKRSRAE, in 1 min at 30°C. Note: 4µg = 6000 units. Ref.: Brophy, M.C., et al. 2006. J. Vasc. Res. 39, 95. Colbran J.L., et al. 1992. J. Biol. Chem. 267, 9589. Corbin, J.D. and Doskeland, S.O. 1983. J Biol Chem 258, 11391. Witt, J.J. and Roskoski, R., Jr, 1975. Anal Biochem 66, 253. |
