Protein Kinase Cm

Liquid. 150 mM NaCl, 50 mM Tris-HCl, 0.25 mM DTT, 0.1 mM EDTA, 0.1 mM EGTA, 0.1 mM PMSF, 25% glycerol, pH 7.5. AVOID FREEZE/THAW CYCLES. Full-length, recombinant, human PKCµ (accession number = NM_X75756) fused at the N-terminus to GST and expressed in S. frugiperda insect cells using a baculovirus expression system. PKCµ is a member of the protein kinase C family that is calcium-independent, phospholipid-dependent, serine/threonine kinase. Specific activity: ≥560 nmol/min/mg protein. Kinase activity is measured as the molar amount of phosphate incorporated into CREBtide using a final concentration of 50 µM ATP (0.83 µCi/assay), 30°C. Purity: ≥90% by SDS-PAGE.

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