LAR-D1
Protein Tyrosine Phosphatase LAR

Liquid. In 100 mM NaCl, 50 mM Tris-HCl, 5 mM DTT, 2 mM EDTA, 0.01% BRIJ^® 35 Detergent, 50% glycerol, pH 7.0. AVOID FREEZE/THAW CYCLES. Recombinant, human PTP-LAR (soluble catalytic domain) expressed in E. coli. Involved in the modulation of insulin receptor signaling in intact cells. Suitable for dephosphorylation of phosphotyrosine residues in proteins. Inhibited by vanadate. Activity: ≥5000 units/ml. Specific activity: ≥5000 units/mg protein. One unit is defined as the amount of enzyme that will hydrolyze 1 nmol of pNPP per min at 30°C, pH 7.0. Purity: ≥95% by SDS-PAGE. Contaminants: DNase activity: <10% conversion to RFII and linear forms as detected by agarose gel electrophoresis; RNase activity: <10% digestion of RNA as detected by agarose gel electrophoresis; Protease activity: None detected; Serine-Threonine Phosphatase activity: None detected.

Ref.: Kulos, D.T., et al. 1995. J. Biol. Chem. 270, 2435. Cho, H., et al. 1991. Biochemistry 30, 6210. Gordon, J.A. 1991. Methods Enzymol. 201, 477. Tsai, A.Y., et al. 1991. J. Biol. Chem. 266, 10534.