Protein Tyrosine Phosphatase 1B

Liquid. In 50 mM HEPES, 1 mM DTT, 1 mM EDTA, 0.05% NP-40, pH 7.2. AVOID FREEZE/THAW CYCLES. Recombinant, human PTP1B expressed in E. coli. PTP1B, an ubiquitous nontransmembrane protein tyrosine phosphatase, was originally identified in human placenta. This highly active enzyme is useful for the study of tyrosine phosphatase kinetics, substrate specificity, and for screening inhibitors. Specific activity: ≥20 units/mg. One unit is defined as the amount of enzyme that will hydrolyze 1 µmol of phosphopeptide substrate (Cat. No. 539737) per min at 30°C, pH 7.2 using 150 µM of substrate. Purity: ≥95% by SDS-PAGE.

Ref.: Puius, Y.A., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 13420. Liu, F., et al. 1996. J. Biol. Chem. 271, 31290.