Protein Tyrosine Phosphatase 1B Assay Kit, Colorimetric | Format: 96-well plate | | Form: 96 Tests | | Detection Method: Colorimetric | | Assay Time: 2 h | | Sample Type: Purified enzyme | | Positive Control: Recombinant PTP1B | | Kit Contents: Human Recombinant PTP1B (Cat. No. 539735), PTP1B Substrate (Cat. No. 539737), Assay Buffer, Phosphate Detection Agent, Phosphate Standard, RK-682 Inhibitor (Cat. No. 557322), ½ Volume Plate, and a user protocol. | | Comments: A colorimetric assay kit to measure PTP1B activity. This convenient 96-well assay is useful for screening inhibitors and modulators of PTP1B activity. | | Ref.: Elchebly, M., et al. 1999. Science 283, 1544. Puius, Y.A., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 13420. Liu, F., et al. 1996. J. Biol. Chem. 271, 31290. Fujii, S., et al. 1995. Neurosci. Lett. 187, 133. Harder, K.W., et al. 1994. Biochem. J. 298, 395. Zhang, Z. Y., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 4446. Martin, B., et al. 1985. J. Biol. Chem. 260, 14932. | | R: 36/38-21/22; S: 26-24/25 | |