2-{[2-bis(Carboxymethyl)amino-5-methylphenoxy]methyl}-6-methoxy-8-bis(carboxymethyl)aminoquinoline, 4K Yellow solid. PROTECT FROM LIGHT. Binding of Ca2+ leads to a major shift in UV absorption spectrum and a twenty-fold enhancement in fluorescence quantum yield. TPEN (Cat. No. 616394) can be used in conjunction with QUIN 2 to reduce heavy-metal quenching of QUIN 2 fluorescence in cells. Purity: ≥98% by UV. Excitation max.: 354 nm (low), 332 nm (high), Emission max.: 510 nm (low), 505 nm (high) CAS 73630-23-6. Ref.: Komada, H., et al. 1989. Cell Struct. Funct. 14, 141. Vijayaraghavan, S. and Hoskins, D. 1989. Cell Calcium 10, 241. Arslan, P., et al. 1985. J. Biol. Chem. 260, 2719. Bryant, D.T. 1985. Biochem. J. 226, 613. Grynkiewicz, G., et al. 1985. J. Biol. Chem. 260, 3440. Ashley R.H., et al. 1984. Biochem. J. 219, 149. Tsien, R.Y., et al. 1982. J. Cell Biol. 94, 325. Tsien, R.Y. 1980. Biochemistry 19, 2396.
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