Liquid. In 100 mM KCl, 20 mM Tris-HCl, 1 mM DTT, 0.2 mM EDTA, 20% glycerol, pH 8.0. AVOID FREEZE/THAW CYCLES. Recombinant, human RAD51 fused at the N-terminus to a His•Tag® sequence and expressed in E. coli. The human RAD51 is a mammalian homologue of yeast RAD51 and bacterial RecA and plays a pivotal role in the homologous recombination pathway for DNA double-strand break (DSB) repair. RAD51 has been shown to colocalize with tumor suppressor proteins BRCA1 (Cat. No. 203659) and BRCA2 in DNA-damage-induced foci and RAD51 is known to interact directly with BRCA2. One unit equals 1 ng of purified protein. 20 units are sufficient for a DNA-protein assay and 100 units are sufficient for a protein-protein interaction assay. Purity: ≥95% by SDS-PAGE.

Ref.: West, S.C. 2003. Nat. Rev. Mol. Cell Biol. 4, 435. Jasin, M. 2002. Oncogene 21, 8981. Symington, L.S., et al. 2002. Microbiol. Mol. Biol. Rev. 66, 630. Daboussi, F., et al. 2002. Cell. Signal. 14, 969. Liu, Y. and West, S.C. 2002. Breast Cancer Res. 4, 9.