Protein Tyrosine Phosphatase SHP-2
Protein Tyrosine Phosphatase 2C Liquid. In 75 mM NaCl, 25 mM Tris-HCl, 10 mM glutathione, 2 mM EDTA, 1 mM DTT, 50% glycerol, 0.05% TWEEN®-20 Detergent, pH 8.0. AVOID FREEZE/THAW CYCLES. Recombinant, human PTP-SHP-2 (amino acids 224-529) fused to the N-terminus to GST and expressed in E. coli. SHP-2 is a regulator of the JAK2 signaling pathway and may also regulate Akt. Plays an important role in growth factor and insulin signaling. Suitable for the dephosphorylation of phosphotyrosine residues in substrate proteins. Specific activity: ≥2 U/µg. One unit is defined as the amount of enzyme that will hydrolyze 1 nmol of pNPP per min at 30°C, pH 7.4. EC 3.3.3.48. Ref.: Chen, J., et al. 2004. Oncogene 23, 3659. Zito, C.I., et al. 2004. J. Cell. Physiol. 199, 227. Ali, S., et al. 2003. J. Biol. Chem. 278, 52021. |
