b-D-Galactosyl-b1,4-N-acetyl-b-D-glucosamine-a2,6-sialyltransferase
a2, 6-NST

Liquid. In 250 mM NaCl, 50 mM MES, 500 µM b-mercaptoethanol, 50% glycerol, pH 6.2. AVOID FREEZE/THAW CYCLES. Soluble, recombinant, human a2,6-(N)-sialyltransferase, (lacking the N-terminal membrane spanning region) fused at the N-terminus to a His•Tag^® sequence and expressed in S. frugiperda insect cells using a baculovirus expression system. Sialylates N-linked oligosaccharides of glycoproteins. Exhibits strict specificity for Galb1,4GlcNAc sequence of oligosaccharides, transferring sialic acid to the 6-position of galactose. Specific activity: ≥1 unit/mg protein. One unit is defined as the amount of enzyme that will transfer 1.0 µmol sialic acid from CMP-sialic acid to N-acetyllactosamine per min at 37°C, pH 7.4. Purity: ≥90% by SDS-PAGE.

Ref.: Williams, M.A., et al. 1995.Glycoconj. J. 12, 755. Colley, K.J., et al. 1989. J. Biol. Chem. 264, 176169.