Sphingomyelin Cholinephosphohydrolase
Sphingomyelin Phosphodiesterase

Liquid. In 250 mM phosphate buffer, 50% glycerol, pH 7.5. Native sphingomyelinase from Staphylococcus species. May be applied exogenously to initiate the sphingomyelin signaling pathway. Has very little activity on phosphatidylcholine. Specific activity: ≥100 units/mg protein. One unit is defined as the amount of enzyme that will hydrolyze 1 µmol of trinitrophenylaminolauryl (TNPAL)-sphingomyelin per min at 37°C, pH 7.4. EC 3.1.4.12, CAS 9031-54-3.

Ref.: Galve-Roperh, I., et al. 1997. Brain Res. Mol. Brain Res. 52, 90. Jarvis, W.D., et al. 1994. Proc. Natl. Acad. Sci. USA 91, 73. Mathias, S., et al. 1993. Science 259, 519. Raines, M.A., et al. 1993. J. Biol. Chem. 268, 14572.