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| | PhosphoDetect™ Anti-Src (pTyr418) Rabbit pAb |
|  | Cat. No. 569373 |
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Anti-pp60src, Phospho-Specific (Tyr418) | 1 T is sufficient antibody for one immunoblot using a 10 ml volume. | | Host: Rabbit | | Isotype: IgG | | Immunogen: a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr418 phosphorylation site of human Src | | Form: Liquid | | Formulation: In Dulbecco's PBS (without Mg2+ and Ca2+), 50% glycerol, 1 mg/ml BSA, pH 7.2-7.4. | | Preservative: ≤0.1% sodium azide | | Positive Control: Chicken embryo fibroblasts (CEF) expressing human wild type src | | Comments: Recognizes the ~60 kDa Src protein phosphorylated at Tyr418 in chick embryo fibroblasts expressing human wild-type Src. | | Ref.: Bourguignon, L.Y.W., et al. 2001. J. Biol. Chem. 276, 7327. Ushio-Fukai, M., et al. 2001. J. Biol. Chem. 276, 48269. |
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EMD Chemicals Inc. list price is displayed (pricing with local distributors may vary). NOTE: In Stock status is based on item availability worldwide.
* Additional Product Information
| | chicken, human, mouse | FS, IB, PS
See Key |
Detection of human Src, phosphorylated on Tyr418, by immunoblotting. Samples: Whole cell lysate from chick embryo fibroblasts cells untransfected (lane 1) or transfected with wild-type human Src (lanes 2-5). Primary antibody: PhosphoDetect™ Anti-Src (pTyr418) Rabbit pAb (Cat. No. 569373) (1:1000) pre-incubated without peptide (lanes 1, 2), with non-phosphopeptide corresponding to the immunogen (lane 3), a generic phosphoserine containing peptide (lane 4), or phosphopeptide immunogen (lane 5). Detection: chemiluminescence.
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