 Detection of mouse STAT1, phosphoylated on Tyr701, by immunoblotting. Samples: Whole cell lysates from 3T3L1 cells left unstimulated (lane 1) or stimulated with LIF (lanes 2-5). Primary antibody: PhosphoDetect™ Anti-STAT1 (pTyr701) Rabbit pAb (Cat. No. 569382) (1:1000) pre-incubated without peptide (lanes 1, 2), with non-phosphopeptide corresponding to the immunogen (lane 3), a generic phosphoserine-containing peptide (lane 4), or the phosphopeptide immunogen (lane 5). Detection: chemiluminescence.
|