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| | PhosphoDetect™ Anti-STAT1 (pSer727) Rabbit pAb |
|  | Cat. No. 569383 |
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| Host: Rabbit | | Isotype: IgG | | Immunogen: a synthetic phosphopeptide corresponding to amino acids surrounding the Ser727 phosphorylation site of human STAT1 | | Form: Liquid | | Formulation: In Dulbecco's PBS, 1 mg/ml BSA, 50% glycerol, pH 7.3. | | Preservative: ≤0.1% sodium azide | | Positive Control: UV-treated HEK293 cells or LIF-treated 3T3-L1 adipocytes. | | Comments: Recognizes STAT1 phosphorylated at Ser727 in uv-treated HEK293 cells and LIF-treated 3T3-L1 cells. | | Ref.: Abe, K. et al. 2001. Oncogene 20, 3464. Kovarik, P., et al. 2001. EMBO J. 20, 91. Kovarik, P., et al. 1999. Proc. Natl. Acad. Sci. USA 96, 13956. Stephens, J.M et al. 1998. J. Biol. Chem. 273, 31408. Ihle, J.N. 1996. Cell 84, 331. Wen, Z., et al. 1995. Cell 82, 241. |
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* Additional Product Information
Detection of human STAT1, phosphorylated on Ser727 by immunoblotting. Samples: Whole cell lysate from HEK293 cells left untreated (lane 1) or treated with UV (lanes 2-5) Primary antibody: PhosphoDetect™ Anti-STAT1 (pSer727) Rabbit pAb (Cat. No. 569383) (1:1000) pre-incubated without peptide (lanes 1, 2), with non-phosphopeptide corresponding to the immunogen (lane 3), a generic phosphoserine-containing peptide (lane 4), or the phosphopeptide immunogen (lane 5). Detection: chemiluminescence.
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