Metablastin
Prosolin Liquid. In 150 mM NaCl, 10 mM phosphate buffer, 1 mM EDTA, pH 7.4. AVOID FREEZE/THAW CYCLES. Recombinant, human stathmin expressed in E. coli. A highly-conserved cytosolic phosphoprotein that acts as a tubulin-sequestering protein via formation of a T2S tight ternary complex. Interferes with the dynamic instability of microtubules in vitro and in vivo. In vitro, it either promotes rapid depolymerization of microtubules or prevents microtubule assembly in polymerization inhibition assays. Specific activity: One mole stathmin will sequester two moles tubulin heterodimer to form a T2S complex; the affinity of stathmin for tubulin is dramatically reduced by the phosphorylation of stathmin. Purity: ≥95% by SDS-PAGE. pI 6.8. Ref.: Curmi, P.A., et al. 1997. J. Biol. Chem. 272, 25029. Jourdain, L., et al. 1997. Biochemistry 36, 10817. Curmi, P.A., et al. 1994. Biochem J. 300, 331. Sobel, A. 1991. Trends Biochem Sci. 16, 301. |
