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| | PhosphoDetect™ Anti-STAT3 (pSer727) Rabbit pAb |
|  | Cat. No. 569392 |
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| Host: Rabbit | | Isotype: IgG | | Immunogen: a synthetic phosphopeptide corresponding to amino acids surrounding the Ser727 phosphorylation site of human STAT3 | | Form: Liquid | | Formulation: In Dulbecco's PBS, 1 mg/ml BSA, 50% glycerol, pH 7.4. | | Preservative: ≤0.1% sodium azide | | Comments: Recognizes the ~90 kDa STAT3 protein phosphorylated at Ser727. | | Ref.: Abe, K., et al. 2001. Oncogene 20, 3464. Stephens, J.M., et al. 1998. J. Biol. Chem. 47, 31408. Ihle, J.N. 1996. Cell 84, 331. Wen, Z., et al. 1995. Cell 82, 241. |
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Detection of mouse STAT3, phosphorylated on Ser727, by immunoblotting. Sample: No peptide (lane 1), the non-phophopeptide corresponding to the immunogen (lane 2), a generic phosphoserine-containing peptide (lane 3) or, the phosphopeptide immunogen (lane 4). Primary antibody: PhosphoDetect™ Anti-STAT3 (pSer727) Rabbit pAb (Cat. No. 569392) (1:1000). Detection: chemiluminescence.
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