| Format: 96-well plate | | Form: 96 Tests | | Detection Method: Colorimetric | | Assay Range: 0.025-0.25 units per ml SOD | | Unit Definition: The amount of enzyme needed to exhibit 50% dismutation of the superoxide radical. | | Assay Time: 1.5 h | | Sample Type: Plasma, serum, erythrocyte lysates, and other lysates, tissue homogenates, and cell lysates | | Positive Control: Bovine erythrocyte SOD (Cu/Zn) | | Kit Contents: Assay Buffer, Sample Buffer, Radical Detector, SOD Standard, Xanthine Oxidase, 96-Well Plate, Plate Cover, and a user protocol. | | Comments: A sensitive spectrophotometric assay (450 nm) for the measurement of all three types (Cu/Zn, Mn, and Fe) of SOD A. | | Ref.: Maier, C.M. and Chan, P.H 2002. The Neuroscientist 8, 323. Mattiazz, M., et al. 2002. J. Biol. Chem. 277, 29626. Beckman, J.S. and Koppenol, W.H. 1996. Am. J. Physiol. 271, C1424. Liu, D. 1996. J. Mol. Neuro. 7, 159. MacMillan-Crow, L.A., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 11853. Sun, E., et al. 1995. Biol. Trace Elem. Res. 48, 231. Sandstrom, J., et al. 1994. J. Biol. Chem. 269, 19163. Marklund, S. 1980. Acta Physiol. Scand. Suppl. 492, 19. Malstrom, B. 1975. in The Enzymes. Boyer, P., editor. XIIB, Academic Press, New York, 533. | |