TATA Box Binding Protein

Liquid. In 100 mM KCl, 20 mM Tris-HCI, 1 mM DTT, 200 µM EDTA, 20% glycerol, pH 7.9. AVOID FREEZE/THAW CYCLES. Recombinant, human TBP fused to a His•Tag^® sequence and expressed in E. coli. The TATA-binding protein (TBP) is believed to function as an essential factor of general transcription machinery and to be involved in transcription by all three eukaryotic RNA polymerases (pol I, II, and III). TBP specifically binds to TATA element at the promoter region and interacts with numerous transcription factors, including TBP-associated factors (TAFs), activators, and some tumor-suppressor proteins. Activity: 1 unit/ng protein. One unit is sufficient for a gel mobility assay in a 20 µl reaction; 10 units are sufficient for a 25 µl reconstituted transcription reaction; and 100 units are sufficient for a protein-protein interaction assay detected by immunoblot system. Note: 1 unit = 1 ng. Purity: ≥95% by SDS-PAGE. Contaminants: DNase, RNase, and protease activity: none detected. Note: 1 KU = 1000 units.

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