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| | PhosphoDetect™ Anti-Tau (pSer199, Ser202) Rabbit pAb |
|  | Cat. No. 577802 |
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| Host: Rabbit | | Isotype: IgG | | Immunogen: a synthetic phosphopeptide corresponding to amino acids surrounding the Ser199 and Ser202 phosphorylation sites of human Tau | | Form: Liquid | | Formulation: In Dulbecco's PBS (w/o Mg2+ and Ca2+) pH 7.3 (± 0.1), 50% glycerol, 1.0 mg/ml BSA (IgG, protease free) as a carrier. | | Preservative: ≤0.1% sodium azide | | Positive Control: Recombinant human tau or CV-1 cells stably expressing human four-repeat tau and SV40 small T antigen. | | Comments: Recognizes the ~45-68 kDa Tau protein phosphorylated at Ser199 and Ser202. | | Ref.: Gong, C.X., et al. 2000. J. Biol. Chem. 275, 5535. Jenkins, S.M., et al. 2000. Biochem. J. 345, 263. Tesseur, I., et al. 2000. Am. J. Pathol. 156, 951. Takahashi, M., et al. 2000. Brain Res. 857, 193. Sontag, E., et al. 1999. J. Biol. Chem. 274, 25490. |
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Detection of human Tau, phosphorylated at Ser199/202, by immunoblotting. Sample: Whole cell lysate from CV-1 cells stably expressing human four-repeat tau and SV40 small T antigen to induce inhibition. Primary antibody: PhosphoDetect™ Anti-Tau (pSer199, Ser202) Rabbit pAb (Cat. No. 577802) (1:000) pre-incubated without peptide (lanes 1), with phosphopeptide immunogen (lane 2), non-phosphopeptide corresponding to the immunogen (lane 3), tau phosphopeptide corresponding to [pS199] (lane 4), tau phosphopeptide corresponding to [pS202] (lane 5), or a generic phosphoserine containing peptide (lane 6). Detection: chemiluminescence.
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