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| | PhosphoDetect™ Anti-Tau (pThr212) Rabbit pAb |
|  | Cat. No. 577810 |
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| Host: Rabbit | | Isotype: IgG | | Immunogen: a synthetic phosphopeptide corresponding to amino acids surrounding the Thr212 phosphorylation site of human Tau | | Form: Liquid | | Formulation: In Dulbecco's PBS, 1 mg/ml BSA, 50% glycerol, pH 7.2-7.4. | | Preservative: ≤0.1% sodium azide | | Positive Control: Recombinant human tau treated with GSK-3b | | Comments: Recognizes the ~45-68 kDa Tau protein phosphorylated at Thr212. | | Ref.: del C. Alonso, A., et al. 2001. J. Biol. Chem. 276, 37967. Sawamura, N., et al. 2001. J. Biol. Chem. 276, 10314. Woods, Y.L., et al. 2001. Biochem. J. 355, 609. Bennecib, M., et al. 2000. FEBS Lett. 485, 87. Jenkins, S.M., et al. 2000. Biochem. J. 345, 263. Maas, T., et al. 2000. J. Biol. Chem. 275, 15733. Takahashi, M., et al. 2000. Brain. Res. 857, 193. Tesseur, I., et al. 2000. Am. J. Pathol. 156, 951. Kopke, E., et al. 1993. J. Biol. Chem. 268, 24374. Arigada, P.A., et al. 1992. Neurology 42, 631. Drubin, D.G. and Kirschner, M.W. 1986. J. Cell. Biol. 103, 2739. |
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Detection of human tau, phosphorylated at Thr212, by immunoblotting. Samples: Whole cell lysate from control cells spiked with exogenously added human recombinant tau left untreated (lane 1) or treated with GSK-3b (lanes 2-5). Primary antibody: PhosphoDetect™ Anti-Tau (pThr212) Rabbit pAb (Cat. No. 577810) (1:000) pre-incubated without peptide (lanes 1, 2), with non-phosphopeptide corresponding to the immunogen (lane 3), a generic phosphoserine containing peptide (lane 4), or phosphopeptide immunogen (lane 5). Detection: chemiluminescence.
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