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Alzheimer's Disease Interactive Pathway and Products
  PhosphoDetect™ Anti-Tau (pSer214) Rabbit pAb
Cat. No. 577811  
All Categories » Calbiochem » Antibodies » Primary Antibodies » Extracellular Matrix and Structural Proteins » Tau/Tubulins

Note: 1 T = 1 test.
Host: Rabbit
Isotype: IgG
Immunogen: a synthetic phosphopeptide corresponding to amino acids surrounding the Ser214 phosphorylation site of human Tau
Form: Liquid
Formulation: In Dulbecco's PBS (without Mg2+ and Ca2+), pH 7.3 (+/- 0.1), 50% glycerol, 1 mg/ml BSA (IgG, protease free).
Preservative: ≤0.1% sodium azide
Positive Control: Human recombinant tau treated with GSK-3b
Comments: Recognizes the ~45-68 kDa Tau protein phophorylated at Ser214.
Ref.: del C. Alonso, A., et al. 2001. J. Biol. Chem. 276, 37967. Sawamura, N., et al. 2001. J. Biol. Chem. 276, 10314. Bennecib, M., et al. 2000. FEBS Lett. 485, 87. Jenkins, S.M., et al. 2000. Biochem. J. 345, 263. Maas, T., et al. 2000. J. Biol. Chem. 275, 15733. Tesseur, I., et al. 2000. Am. J. Pathol. 156, 951. Jicha, G.A., et al. 1999. J. Neurosci. 19, 7486. Schneider, A., et al. 1999. Biochemistry 38, 3549. Hoffmann, R. et al. 1997. Biochemistry 36, 8114. Kopke, E., et al. 1993. J. Biol. Chem. 268, 24374. Arigada, P.A., et al. 1992. Neurology 42, 631. Drubin, D.G. and Kirschner, M.W. 1986. J. Cell. Biol. 103, 2739.

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10 TYN/A

CloneSpecies ReactivityApplication
 humanIB 
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Detection of human Tau, phosphorylated at Ser214, by immunoblotting. Samples: Human recombinant Tau untreated (lane 1) or treated with GSK-3b (1 µg per µg Tau, for 45 min) (lanes 2-5). Primary antibody: PhosphoDetect™ Anti-Tau (pSer214) Rabbit pAb (Cat. No. 577811) (1:1000) pre-incubated without peptide (lanes 1, 2), with non-phosphopeptide corresponding to the immunogen (lane 3), a generic phosphoserine containing peptide (lane 4), or phosphopeptide immunogen (lane 5). Detection: chemiluminescence.

Related Literature:

Alzheimer's Disease Brochure & Technical Guide

PhosphoDetect™ Phospho-specific Antibodies Brochure

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577811: PhosphoDetect™ Anti-Tau (pSer214) Rabbit pAb - English
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Related Categories:
All Categories » Calbiochem » Antibodies » Primary Antibodies » Extracellular Matrix and Structural Proteins » Tau/Tubulins