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Alzheimer's Disease Interactive Pathway and Products
  PhosphoDetect™ Anti-Tau (pSer396) Rabbit pAb
Cat. No. 577815  
All Categories » Calbiochem » Antibodies » Primary Antibodies » Extracellular Matrix and Structural Proteins » Tau/Tubulins

Host: Rabbit
Isotype: IgG
Immunogen: a synthetic phosphopeptide corresponding to amino acids surrounding the Ser396 phosphorylation site of human Tau
Form: Liquid
Formulation: In Dulbecco's PBS (without Mg2+ and Ca2+), 1 mg/ml BSA, 50% glycerol, pH 7.2-7.4.
Preservative: ≤0.1% sodium azide
Positive Control: Recombinant human tau treated with GSK-3b
Comments: Recognizes the ~45-68 kDa Tau protein phosphorylated at Ser396.
Ref.: del C. Alonso, A., et al. 2001. J. Biol. Chem. 276, 37967. Bennecib, M., et al. 2001. FEBS Lett. 490, 15. Sawamura, N., et al. 2001. J. Biol. Chem. 276, 10314. Tomidokoro, Y., et al. 2001. Neurosci. Lett. 299, 169. Bennecib, M., et al. 2000. FEBS Lett. 485, 87. Hashiguchi, M., et al. 2000. J. Biol. Chem. 275, 25247. Jenkins, S.M., et al. 2000. Biochem. J. 345, 263. Maas, T., et al., 2000. J. Biol. Chem. 275, 15733. Takahashi, M., et al. 2000. Brain. Res. 857, 193. Tesseur, I., et al. 2000. Am. J. Pathol. 156, 951. Schneider, A., et al. 1999. Biochemistry 38, 3549. Sengupta, A., et al. 1998. Arch. Biochem. Biophys. 357, 299. Wang, J.G., et al. 1998. FEBS Lett. 436, 28. Kopke, E., et al. 1993. J. Biol. Chem. 268, 24374. Arigada, P.A., et al. 1992. Neurology 42, 631. Drubin, D.G. and Kirschner, M.W. 1986. J. Cell. Biol. 103, 2739.

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EMD Chemicals Inc. USD list price is displayed (pricing with local distributors may vary). NOTE: In Stock status is based on item availability worldwide.

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10 TY$343.00*

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Cat. No.Disclaimers
577815
Canadian Export RegulationsDue to the country and/or U.S. state of origin of the animal material used in this product, this product may not be exported to Canada.

CloneSpecies ReactivityApplication
 humanIB 
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Detection of human tau, phosphorylated at Ser396, by immunoblotting. Samples: Whole cell lysate from control cells with exogenously added human recombinant tau left untreated (lane 1) or treated with GSK-3b (lanes 2-5). Primary antibody: PhosphoDetect™ Anti-Tau (pSer396) Rabbit pAb (Cat. No. 577815) (1:1000) pre-incubated without peptide (lanes 1, 2), with non-phosphopeptide corresponding to the immunogen (lane 3), a generic phosphoserine containing peptide (lane 4), or phosphopeptide immunogen (lane 5). Detection: chemiluminescence.

Available Separately:
525282: PhosphoDetect™ Phosphoserine Detection Kit
525288: PhosphoDetect™ Phosphothreonine Detection Kit
525322: PhosphoDetect™ Phosphotyrosine Detection Kit

May We Suggest:
71296: PhosphoSafe™ Extraction Reagent

Related Literature:

Alzheimer's Disease Brochure & Technical Guide

PhosphoDetect™ Phospho-specific Antibodies Brochure

Material Safety Data Sheets:
577815: PhosphoDetect™ Anti-Tau (pSer396) Rabbit pAb - English
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Related Categories:
All Categories » Calbiochem » Antibodies » Primary Antibodies » Extracellular Matrix and Structural Proteins » Tau/Tubulins