Pancreatic Trypsin Inhibitor
Trypsin-Kallikrein Inhibitor White to off-white crystalline solid. HYGROSCOPIC. A competitive and reversible inhibitor of esterases and proteases. Forms a tight complex with and blocks the active site of the enzymes. Inhibits a number of different proteases, including chymotrypsin, coagulation factors involved in the prephase of blood clotting, kallikrein (Kd = 1 x 10-7 M), plasmin (Kd = 2.3 x 10-10 M) , tissue and leukocytic proteinases, and trypsin (Kd = 5 x 10-14 M). Does not inhibit Factor Xa and thrombin. It is relatively acid- and heat-stable. Useful as a serine protease inhibitor during purification of proteins and in studies of zymogen activation systems. Activity: ≥5500 KIU/mg dry weight. One Kallikrein Inhibitory Unit (K.I.U.) is defined as the quantity of protease inhibitor that will inhibit two kallikrein units by 50% under optimal conditions. Note: 1 KIU = 0.025 antiplasmin units (APU) or 0.0031 trypsin inhibitor units. Purity: ≥95% by gel filtration. Contaminants: endotoxin: ≤10 EU/mg. Effectively inhibits target proteases at equimolar concentration. pH optimum 5 - 7, pI 10.5. RTECS YN5080000, CAS 9087-70-1. Note: 1 K.I.U. = 0.025 antiplasmin units (APU) or 0.0031 trypsin inhibitor units. Merck Index: 14, 757
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