Apo2L, Human, Recombinant, E. coli TNF-Related Apoptosis-Inducing Ligand, Human, Recombinant, E. coli Liquid. In 500 mM NaCl, 10 mM Na2HPO4, 2.7 mM KCl, 2 mM KH2PO4, 0.1 mM DTT, ≤10% glycerol. AVOID FREEZE/THAW CYCLES. Recombinant, human TRAIL (amino acids 114-281) expressed in E. coli. Ligand for TRAIL receptors DR4 and DR5. Displays potent antitumor activity against selected targets and induces apoptosis in a number of transformed cell lines, but not in normal tissues. Biological activity: Induces apoptosis in U937 cells at 1 mg/ml within 5 h.. Purity: ≥95% by SDS-PAGE. Contaminants: endotoxin <0.1 EU/µg TRAIL. Ref.: Lin, Y., et al. 2000. Mol. Cell. Biol. 20, 6638. Mitsaides, N., et al. 2000. Cancer Res. 60, 4122. Griffith, T.S., et al. 1999. J. Exp. Med. 189, 1343. Mariani, S.M., et al. 1997. J. Cell Biol. 137, 221. Pan, G., et al. 1997. Science 276, 111. Pan, G., et al. 1997. Science 277, 815. Sheridan, J.P., et al. 1997. Science 277, 818. Pitti, R.M., et al. 1996. J. Biol. Chem. 271, 12687. Wiley, S.R., et al. 1995. Immunity 3, 673. |