Apo2L; TNF-Related Apoptosis-Inducing Ligand Lyophilized. Lyophilized from sterile-filtered PBS, 50 µg BSA/µg TRAIL, pH 7.4. Recombinant, human TRAIL expressed in NSO cells a type II transmembrane protein with a C-terminal extracellular domain, which exhibits homology to other TNF family members. Displays potent antitumor activity against selected targets. Induces apoptosis in a number of transformed cell lines but not in normal tissues. Biological activity: ED50 = 4-12 ng/ml as measured by its ability to induce cytotoxicity in mouse L-929 cell line in the presence of metabolic inhibitors actinomycin D. Purity: ≥97% by SDS-PAGE. Contaminants: Endotoxin: ≤1 EU/µg TRAIL. Ref.: Griffith, T.S., et al. 1999. J. Exp. Med. 189, 1343. Mariani, S.M., et al. 1997. J. Cell Biol. 137, 221. Pan, G., et al. 1997. Science 277, 815. Pan, G., et al. 1997. Science 276, 111. Sheridan, J.P., et al. 1997. Science 277, 818. Pitti, R.M., et al. 1996. J. Biol. Chem. 271, 12687. |
