Solid. Salt-free PROTECT FROM MOISTURE. Native trypsin from bovine pancreas. Serine protease that specifically hydrolyzes amides, peptides and proteins at the C-terminal side of arginine and lysine residues. Inhibitors include aprotinin, DFP, and soybean trypsin inhibitor. Note: 1 MU = 1,000,000 units. Activity: ≥2,500 USP units/mg dry weight. One USP unit is defined as the amount of enzyme that will digest BAEE and cause an increase in absorbance of 0.003 units at 253 nm per min at 25°C, pH 7.6. Contaminants: Chymotrypsin: ≤50 NF units/mg dry weight. pH optimum 8.5 - 8.8. EC 3.4.21.4, RTECS YN5075000, CAS 9002-07-7. Note: 1 NF unit = 1 USP unit = 3 SU at 30°C, pH 7.6; 1 TAME unit = 19.2 USP units = 57.5 BAEE units; 1 MU = 1,000,000 units. Merck Index: 14, 9795
Ref.: Bergmeyer, H.U. 1983. Methods of Enzymatic Analysis, 3rd ed. Vol. 3, 17. Kostka, V., and Carpenter, F.H. 1964. J. Biol. Chem. 239, 1799. Hummel, B.C.W. 1955. Can. J. Biochem. Physiol. 37, 1393.
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